Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello Giovanna

If you search the CONFOCAL archives from earlier this year you will find
quite a bit of discussion about what can and can't be done in this area
with the latest range of confocals. The overall conclusion was, I think,
yes it can be done, but the microscopes are expensive and you probably
need to very very careful in setting up and interpretting your
multi-labels...

IAN


Giovanna Borsellino wrote:
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello,
>
> I have a very limited experience with confocal microscopy (or with any kind
> of microscopy), but in our lab we use 9-colour flow cytometry to
> characterize T cell subpopulations. We would like to purchase a new Confocal
> microscope, and we were wondering whether it would be possible to use 5 or 6
> (or 7, or 8...) fluorochromes. Is it possible? Is there a software which
> will permit to perform "compensation" of loaded images?
>
> Thank you for any suggestion
>
> Giovanna
> -------------------------------------------------------
> Giovanna Borsellino, M.D., Ph.D.
> Neuroimmunology
> IRCCS Santa Lucia
> Via Ardeatina 354
> 00179 Roma
> Italy
>
> Tel. ++39.06.51501521 (office)
>        ++39.06.51501552 (lab)
> Fax  ++39.06.51501553
>
> e-mail: [log in to unmask]
>            [log in to unmask]

--
Professor Ian Gibbins
Anatomy & Histology
Flinders University of South Australia
GPO Box 2100, Adelaide, SA 5001
Australia

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