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We have a PE Ultraview system with the excitation and emission filters controlled via the PE software. The filter wheel is a Sutter Lambda 10-2; 55 msec between adjacent filter positions according to the spec! The excitation wheel sits on the yokagawa scan head - replacing the aforementioned sliders. I'm not sure when this modification came in. It does it's job well, we do not seem to have any problem with our 488 and 568 nm lines with regard to alignment.
Regards
Tony
Senior Research Associate
Laboratory of Molecular Signalling
Babraham Institute
Babraham
Cambridge, UK
CB2 4AT
Tel: +44 (0)1223 496499
Fax: +44 (0)1223 496043
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> -----Original Message-----
> From: Michal Kozubek [SMTP:[log in to unmask]]
> Sent: 18 November 2002 14:18
> To: [log in to unmask]
> Subject: Re: dedicated Live Cell Imaging Systems
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> On Fri, 15 Nov 2002, Alison North wrote:
>
> > 6) ... My current PE
> > system can either do GFP, Rhod and Cy5 together, OR
> CFP and YFP
> together,
> >
> > 7) ... My PE system consists of
> > a Zeiss microscope, the PE head and lasers etc.,
> and then a load
> of filter
> > wheels, cameras, software etc. from Universal Imaging,
>
> Dear Alison,
>
> Thank you very much for your nice comparison! Just
> one question:
> Have you
> managed to automate filter swicthing on your PE
> system using filter
> wheels? Where is the filter wheel situated (in your
> optical path)?
>
> We have also got a Yokogawa CSU-10 system (which PE
> calls Ultraview)
> and have struggled with its automation some time ago. Without
> success.
> The confocal head contains a slider with 3 positions
> for 25mm exc.
> filters
> (e.g. GFP+Rhod+Cy5) but it is very hard to replace it with a
> computer
> driven filter wheel (without drilling into the confocal head).
>
> Putting the filter wheel in front of the laser seems
> to cause severe
> alignment problems (filter surfaces will never be
> parallel to each
> other
> and will cause the light from the multiple-wavelength
> multi-mode
> laser
> miss the 4 micron hole in the optical fiber for
> certain filters).
>
> Another possibility is to put an AOTF between the
> laser and the
> conf. head
> (fiber input and fiber output) but AOTF is very light
> inefficient
> (some
> 30-40% light throughput according to the specifications).
>
> Thanks in advance for advice,
> best regards,
> Michal
>
> P.S. Which laser (what laser power) do you use with
> your PE for
> GFP/Rhod/Cy5?
> We purchased a Melles-Griot one (Ar/Kr 35 LTL 835) with
> 10/10/15 mW
> for 488/568/647 nm, respectively (the strongest
> multiple-wavelength
> multi-mode laser from Melles-Griot) but find it
> quite week for
> most
> applications. The other possibility we found was Coherent
> Innova 70C
> (2.5W) but nothing in between these two extremes.
>
> Experience and ideas of other listers are also welcome.
>
>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> ~~~~~~~~~~~~~~
> ~~
> Michal Kozubek, Ph.D.
> Laboratory of Optical Microscopy, Faculty of Informatics,
> Masaryk University, Botanicka 68a, CZ-60200, Brno,
> Czech Republic
> Tel/Fax/Ans: +420-5-41512467 E-mail: [log in to unmask]
> Internet home page: http://www.fi.muni.cz/~kozubek
>
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