CONFOCALMICROSCOPY Archives

November 2002

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Subject:
confocal resolution
From:
Kathy Spencer <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 27 Nov 2002 12:32:17 -0800
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello All!
         Basic generic question....what is the limit of resolution on a
confocal? I'm using an Olympus Fluoview confocal, 60x 1.4NA objective, oil
(1.516), pinhole automatically set for 1 Airy disk. For 488nm laser, I
calculate 174nm using resolution=wavelength/2NA. But can the confocal
resolve more than that? What is a good formula?
         Thanks!
         Kathy








Kathy Spencer
The Scripps Research Institute
10550 N. Torrey Pines Road
ICND 202
La Jolla, CA 92037
858-784-8437

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