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Hi

we had to think along the same lines while preparing for a FRAP
experiment, so I thought I would just check with you all.  What happens
to the size of the spot size on the specimen when you go from 63/1.4 to 100/1.4?

thanks

Jose Feijo wrote:
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> hi benedikt:
> resolution should be the same if the NA is the same. There are advantadges in using a 60 (or 63): wider field and usually much better transmitance (back apertures are usually much larger) are two that come to mind.
>
>
> *********** REPLY SEPARATOR ***********
> On 11/27/2002 at 6:52 PM Benedikt Kost wrote:
> am I better off with a  63x /na 1,4 (oil immersion) or with a 100x/na 1,4 (oil immersion) lens for the confocal imaging of small cells with a diameter of around 10 micrometer, given that through the 63x lens I can scan a ca 7.5x7.5 micrometer square at maximal zoom?
>
> thanks a lot for your comments!
>
> benedikt kost
>
> ----------------------------------------------------------------------
> Benedikt Kost, PhD
> Heidelberg Institute for Plant Sciences (HIP)
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