Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
It is possible to do FRET with any confocal microscope. However, it is
very important to correct the spectral bleedthrough signal. The following
paper describes how to do this:
M. Elangovan, H. Wallrabe, Y. Chen, R. Day, M. Barroso, A. Periasamy.
2003. Characterization of one- and two-photon excitation fluorescence
resonance energy transfer microscopy. Methods. 29: 58-73.
CircuSoft Instrumentation also has software that performs the PFRET
algorithm described above. It can be used with 8-bit tiff images from any
confocal (or wide field, two-photon) setup. For more information, please
see http://www.circusoft.com/fret.html or send me an email.
Sincerely,
Daniel Tyreus
CircuSoft Instrumentation