Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

This is an appealing approach, too:

Karpova TS, Baumann CT, He L, Wu X, Grammer A, Lipsky P, Hager GL, McNally
JG. Fluorescence resonance energy transfer from cyan to yellow fluorescent
protein detected by acceptor photobleaching using confocal microscopy and a
single laser. J Microsc. 2003 Jan;209(Pt 1):56-70.

I would think the newer scopes with good ROI and data export function should
be particularly useful.  Indeed, it would see likely that the confocal
companies would want to include a FRET module (for a modest fee, of course).

I've seen some suggestion that between AOTF control and spectral imaging
choices, FRET should be a whole lot easier.  Anyone agree (or have practical
experience doing so)?

Mike Mancini

>===== Original Message From Confocal Microscopy List
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>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>It is possible to do FRET with any confocal microscope. However, it is
>very important to correct the spectral bleedthrough signal. The following
>paper describes how to do this:
>
>M. Elangovan, H. Wallrabe, Y. Chen, R. Day, M. Barroso, A. Periasamy.
>2003. Characterization of one- and two-photon excitation fluorescence
>resonance energy transfer microscopy. Methods. 29: 58-73.
>
>CircuSoft Instrumentation also has software that performs the PFRET
>algorithm described above. It can be used with 8-bit tiff images from any
>confocal (or wide field, two-photon) setup. For more information, please
>see http://www.circusoft.com/fret.html or send me an email.
>
>Sincerely,
>Daniel Tyreus
>CircuSoft Instrumentation