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Dear All,

We have a Zeiss LSM 510 setup, version 2.5. We recently acquired a 100x
objective (plan-neofluar 100x/1.3 oil). We are unable to do Z sectioning
using this objective, and always get the same error message
"Range exceeds limit of 1um for plan-neofluar 100x/1.3 objective. Reduce
slices and interval"
If I reduce the stack size to less then 1 um then it will do the z section,
but obviously this is not much use.
What is going on?

A second problem we have with both the 40x and 100x oil objectives, is that
the transmission DIC images are full of the black spots and streaks, which
are consistent every time we scan. The Zeiss technician has taken apart and
cleaned everything he can in the beam path, but there is no improvement. Any
suggestions will be gratefully received.

Thanks,

 Rebecca Haffner, Ph.D
 Dept. of Veterinary Resources,
 Weizmann Institute,
 Rehovot, Israel.
 972 8 9342719 (fax 972 8 9342823)
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