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Dear Listers,

thanks to all who replied to my recent posting concerning Ar/Kr lasers!
Now I have a different question which is not strictly confocal but maybe
someone of you can give me advice. I have until now unsuccessfully tried
to measure pH shift in phagosomes by feeding Molecular Probes'
LysoSensor Yellow/Blue dextran to macrophages and follow acidification
using two filtersets, one ex 365 nm/em 535 nm for lower pH, the other
filterset consists of ex 340 nm/em 460 nm for higher pH, light source is
a xenon lamp. I see nothing although I use a highly sensitive camera.

What is wrong??

Volker