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Hello,

I am interested in estimating the volume density of mitochondria in brine
shrimp embryos. The shell around the encysted embryos makes it difficult to
fix the embryos for EM, so I am looking for alternative methods. Somebody
in my lab suggested that I squish the embryos, label the mitochondria with
MitoTracker and use a point counting method to estimate # of mitochondria
in images taken with a confocal microscope.

Has anyone else tried anything remotely like this? If so do you have any
words of wisdom? So far I am having only limited success with this
technique.


Any assistance will be greatly appreciated.

Julie


Julie Reynolds
Graduate Research Assistant
Dept. of Biological Sciences
Louisiana State University
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