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Hi everyone,

this question is not really a confocal related question, but I thought
someone out there might still be able to help. I have tried to stain the
nuclei of  Caco-2 cells grown on polycarbonate filters using DAPI. The
dye seems to bind to the filter or the filter has a high fluorescence in
the UV range. At least I get a very weak specific signal and a very
brigth background. I have used the same protocol with coverglass grown
cells and got perfect results. Does anyone experienced something similar
and can suggest solutions to this problem (special protocol, other
dyes...)? I would be very grateful for any kind of help.

Daniel Scharlau

--
Daniel Scharlau
Max-Planck Institute for Molecular Physiology
Dep. Epithelial Cell Physiolgy

Otto-Hahn-Str. 11
44227 Dortmund
Germany

Phone: +49 (231) 133-2204
Fax:   +49 (231) 133-2299
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