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Dear Daniel,

A group here counts bacteria stained with DAPI on black filters, which
don't pick up the stain.  They're available from SPI http://www.2spi.com/
and other suppliers.
cheers,
Rosemary

>
>Try SYTO 13 from Molecular Probes. I have used it for nuclear staining of
>Caco-2 cells on polycarbonate filters with a very good result.
>
>Göran Ocklind
>
>-----Ursprungligt meddelande-----
>Från: Confocal Microscopy List [mailto:[log in to unmask]] För
>Daniel Scharlau
>Skickat: den 22 april 2003 09:54
>Till: [log in to unmask]
>Ämne: DAPI on filters!
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi everyone,
>
>this question is not really a confocal related question, but I thought
>someone out there might still be able to help. I have tried to stain the
>nuclei of  Caco-2 cells grown on polycarbonate filters using DAPI. The
>dye seems to bind to the filter or the filter has a high fluorescence in
>the UV range. At least I get a very weak specific signal and a very
>brigth background. I have used the same protocol with coverglass grown
>cells and got perfect results. Does anyone experienced something similar
>and can suggest solutions to this problem (special protocol, other
>dyes...)? I would be very grateful for any kind of help.
>
>Daniel Scharlau
>
>--
>Daniel Scharlau
>Max-Planck Institute for Molecular Physiology
>Dep. Epithelial Cell Physiolgy
>
>Otto-Hahn-Str. 11
>44227 Dortmund
>Germany
>
>Phone: +49 (231) 133-2204
>Fax:   +49 (231) 133-2299
>E-mail: [log in to unmask]


Dr Rosemary White                        [log in to unmask]
Microscopy Centre                        fax     61- 2 6246 5000
CSIRO Plant Industry                      ph.     61- 2 6246 5475 or
GPO Box 1600                              mob.  61- 0402 835 973
Canberra, ACT 2601, Australia