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April 2003

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Confocal Microscopy List <[log in to unmask]>
Subject:
autofluorescence in 293 cells
From:
Patricia Conrad <[log in to unmask]>
Date:
Tue, 29 Apr 2003 14:31:44 -0400
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Has anyone had experience getting rid of autofluorescence from 293
cells?  Two of my colleagues are trying to perform immunofluorescence on
293 cells transfected with WISP3 and LRP5 respectively.  However, the
control cells and transfected cells have high levels of
autofluorescence.  Has anyone else experienced this with this cells
line?  Any suggestions on how to get rid of the background staining?
Both colleagues see this with paraformaledehyde and methanol fixation
protocols, and the cells have been blocked with BSA and/or serum.
Thanks,

Patty Conrad
621 BRB/Dept. of Genetics
CWRU School of Medicine
10900 Euclid Ave
Cleveland, Ohio 44106-4955

Phone:  (216) 368-0305
FAX:    (216) 368-3432

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