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April 2003

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Confocal Microscopy List <[log in to unmask]>
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Wed, 30 Apr 2003 05:53:37 +0300
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<p05200f41bad48ae918c8@[144.92.238.208]> from "James Pawley" at Apr 29, 2003 03:13:49 PM
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"Aryeh M. Weiss" <[log in to unmask]>
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>
> A partial solution can be found in the color CCD cameras that are now
> often found on scopes. Even consumer-grade CCD cameras have fairly
> uniform color response. You can use such a camera to find the color
> of your auto-fluorescence under different excitation conditions.
>
> Images from these camera can tell some surprising tales.  I recently
> detected a poor barrier filter by finding some red areas in a
> fluorescence image that was supposed only to be blue. With a
> monochrome camera, I  would have assumed that all the detected
> photons were blue.
>

However, one must note that the color separation filters in RGB cameras
are fairly broad and not designed for the ultimate in out-of-band rejection.
So if the phenomenon described above is seen, it might be a good idea to
confirm the diagnosis with a spectrophotometer.

--aryeh

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