Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Have a look at the Varispec filters from CRI
http://www.cri-inc.com/instruments/products/index.shtml

No commercial interest, just something I've looked at.

Robert.

-----Original Message-----
From: Ian Clements [mailto:[log in to unmask]]
Sent: 29 April 2003 23:44
To: [log in to unmask]
Subject: Spectral imaging with a camera system


Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Not entirely confocal but some of Jim's comments on autofluoresence got
me
curious.

I was wondering if anyone knew of a image splitter device, like those
from
Optical Insights, being used for performing spectral imaging.
Conceivably if
you equipped the image splitter with appropriate filters, perhaps 20 nm
apart,
you could capture 2-4 images on a single CCD camera (I think they have
such a
configuration).

With appropriate software for linear unmixing analysis you could have
something
analogous to the META system but one that could be configured for
different
wavelength ranges by swapping the filters.  Depending on the amount of
light
lost you could have a relative fast, reasonable resolution spectral
imager, it
might be a good setup for a spinning disk system..

I dare say the same could be acheived with an emission filter wheel but
it would
be somewhat slower.

I'd be interested in hearing about other spectral imaging devices anyone
has had
experience with.  I'm familar with Lightform's Pariss, the Zeiss META,
Leica's
SP system and BioRad's Rainbow(?).

If I'm overlooking something obvious in the above please let me know.

No particular connection with any of the above companies, just looking
to stay
informed.

Ian Clements
Imaging Technology Group
Molecular Probes Inc


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