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April 2003

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Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 7 Apr 2003 23:57:06 +1000
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Confocal Microscopy List <[log in to unmask]>
Subject:
Re: Zoom problem
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From:
Guy Cox <[log in to unmask]>
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Quoting Dr Pedro J Camello <[log in to unmask]>:

> I´m using a BioRad 1024 equipped with a 100 mW Ar laser. While imaging
> Ca2+ signals of fluo-4-loaded smooth muscle cells we found that using a
> 3x digital zoom we were unable to get even small responses. Reducing
> the zoom below 2x resolved the problem, and cells started to give perfect
> responses. We did not see big fluorescence bleaching
>
> Any explanations? :(

You killed your cells is the only thing I can suggest!

                                                         Guy


--
Associate Professor Guy Cox
Electron Microscope Unit, F09
University of Sydney NSW 2006
+61 2 9351 3176

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