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April 2003

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Confocal Microscopy List <[log in to unmask]>
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From:
Terry Wu <[log in to unmask]>
Date:
Tue, 8 Apr 2003 08:52:13 -0700
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Hello Everyone,

Can anyone recommend a software or method to normalize image brightness for a confocal
image series?  We have been collecting large stacks of images (about 100 images/stack)
and photo-bleaching progressively reduces the brightness of images.  We hope that we can
normalize these images before reconstructing them in 3D.

Thanks in advance for your help,

Terry

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