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Sarah;

I think the bottoms of the Nunc chambers are plastic, but have good
optical properties.  

The "strips" contain four chambers.  

The loss of resolution from LWD optics and thick plastic is a pretty
high price to pay if you can solve the problem otherwise.

Tom

-----Original Message-----
From: Sarah Locknar [mailto:[log in to unmask]] 
Sent: Wednesday, April 09, 2003 8:37 AM
To: [log in to unmask]
Subject: plastic on inverted scope


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Hi-
I have a couple of users who want to image cells grown in plastic
microwell plates on an inverted scope.  Of course, the plastic is too
thick to image through, but when I suggested that they grow them on
glass coverslips they replied that the cells don't stick as well and
grow differently on similarly coated glass.  Is it possible to buy
microwell plates with thinner plastic bottoms, and where would I buy
such a thing?  In previous postings I got the feeling that the only
available ones had glass coverslip bottoms.  Also, would the images
suffer greatly when imaging through plastic versus glass?  Would a
better option be to just get a longer-working distance lens? Thanks in
advance- Sarah

------------------------------------------------------------
Sarah Locknar, Ph.D.
Director, Neuroscience COBRE Imaging/Physiology Core
University of Vermont
802-656-0413
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