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IP Lab Spectrum from Scanalytics also will normalise a stack of images
like this

IAN


Scott Monroe wrote:
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> As a imaging software Vendor we use Metamorph or Metavue, they have an
> Equalize function.  Briefly the function can take the average, minimum, or
> maximum light intensity in each plane, or an ROI in an image stack set it to
> equal the average, minimum, or maximum intensity, respectively.
>
> Scott Monroe, Ph.D.
> Nikon Instruments Inc.
>
> ----- Original Message -----
> From: "Tobias" <[log in to unmask]>
> To: <[log in to unmask]>
> Sent: Tuesday, April 08, 2003 11:18 AM
> Subject: Re: How to Normalize Image Series
>
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > > Can anyone recommend a software or method to normalize image
> > > brightness for a confocal
> > > image series?  We have been collecting large stacks of images (about
> > > 100 images/stack)
> > > and photo-bleaching progressively reduces the brightness of images.
> > > We hope that we can
> > > normalize these images before reconstructing them in 3D.
> > >
> >
> > i use bleach_cor, part of the xcosm package. requires unix/linux
> > system. but works quite fine for me. using the same algorithm
> > (non-linear regression.. fairly straightforward i guess) should be
> > possible to write a imagej plugin. anybody did already?
> >
> > tobias
> >

--
Professor Ian Gibbins
Anatomy & Histology
Flinders University of South Australia
GPO Box 2100, Adelaide, SA 5001
Australia

Phone:  +61-8-8204 5271
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