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Another possibility is glow-discharging the glass, which makes it very
attractive to cells.

Lesley Weston.


on 09/04/2003 9:16 AM, Michael Herron at [log in to unmask] wrote:

<snip>
>
> It is sometimes possible to get finicky cells to grow "on glass" if you
> coat the glass (various gelatins, collagens, fibronectin etc.) OR if you
> seed the glass with high concentrations of cells.
>
> Mike
>
> Sarah Locknar wrote:
>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hi-
>> I have a couple of users who want to image cells grown in plastic
>> microwell plates on an inverted scope.  Of course, the plastic is too
>> thick to image through, but when I suggested that they grow them on
>> glass coverslips they replied that the cells don't stick as well and
>> grow differently on similarly coated glass.  Is it possible to buy
>> microwell plates with thinner plastic bottoms, and where would I buy
>> such a thing?  In previous postings I got the feeling that the only
>> available ones had glass coverslip bottoms.  Also, would the images
>> suffer greatly when imaging through plastic versus glass?  Would a
>> better option be to just get a longer-working distance lens?
>> Thanks in advance-
>> Sarah