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Another technique you might want to try involves etching coverslips with IM
HCl.  Cells seem to stick better to glass treated with HCl.  The protocol
follows:


Preparing Acid Washed Glass Cover slips
This helps cells and polyamino acids stick to glass.  It also helps your DIC
pictures look prettier by getting rid of small particles stuck on the glass.

First separate cover slips  from one another and place them into a glass
container with a lid containing the amount of water you'll need to add in
order to make a 1M HCl solution.  It is important that the cover slips are not
sticking to each other because you will need to use one at a time and it's
easier to do this now rather than later.

1) Heat cover slips in a loosely covered glass beaker in 1M HCl at 50-60oC for
4-16h.
2) Cool to room temperature
3) Rinse out 1M HCl with ddH2O
4) Fill container with ddH2O and sonicate in water bath for 30 mins
5) Repeat step 4
6) Repeat step 4
7) Fill container with 50% EtOH and 50% ddH2O and sonicate in water bath for
30 min
8) Fill container with 70% EtOH and 30% ddH2O and sonicate in water bath for
30 min
9) Fill container with 95% EtOH and sonicate in water bath for 30 min
10) Fill container with 95% EtOH


Good luck!

Mike

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Michael C. Adams
Research Technician for Dr. Clare Waterman-Storer
Laboratory of Cell Motility Studies
Department of Cell Biology
The Scripps Research Institute
10550 North Torrey Pines Road, CB 163
La Jolla, CA 92037

TEL 858.784.9244
FAX 858.784.7521
EMAIL [log in to unmask]
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