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We create up to 16 images in every optical x-y plane to
form one optical slice and we have 20 optical slices (planes) (i.e.
every 5 microns).  We save images as tiff but bitmap would be ok.

Thanks for your help,
Ioana Coman

----- Original Message -----
From: Klughammer - Anneliese Schmaus <[log in to unmask]>
Date: Wednesday, April 23, 2003 3:45 am
Subject: Re: 3D object rendering from confocal microscopy images

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> How many images do you create in X-Y direction
> and how many slices in Z-direction in order to render a
> 10 mm x 10 mm x 100 um 3D image? Which size and which
> format (jpg, bmp etc.) does one image have?
>
> mfg / regards
>
> Anneliese Schmaus
> Product Manager
>
> klughammer gmbh
> Strassbach 9
> 85229 Markt Indersdorf
> Germany
> Tel. +49 08136 6011
> Fax  +49 08136 7098
> [log in to unmask]
> www.klughammer.de
>
> IC> Search the CONFOCAL archive at
> IC> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> IC> Does anybody know about an automated program to combine multiple
> IC> physical sections (say 100 micron thick) of the same sample
> (say 10 mm
> IC> in diameter and 10 mm long)  to create a single 3D object (10
> mm in
> IC> diameter and 10 mm long). Each section is imaged with confocal
> IC> microscopy with high resolution, so the whole 3D object is
> renderedIC> with the same high resolution.
>
> IC> Thanks in advance.
> IC> I. Coman
>