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Patricia Conrad wrote:

> Has anyone had experience getting rid of autofluorescence from 293
> cells?  Two of my colleagues are trying to perform immunofluorescence on
> 293 cells transfected with WISP3 and LRP5 respectively.  However, the
> control cells and transfected cells have high levels of
> autofluorescence.  Has anyone else experienced this with this cells
> line?  Any suggestions on how to get rid of the background staining?
> Both colleagues see this with paraformaledehyde and methanol fixation
> protocols, and the cells have been blocked with BSA and/or serum.

Dear Patty--

What color is the autofluorescence?  Does it show up under all
filters--UV through CY5?  The color will give a clue to what the cause
might be.

Good luck--

Martin Wessendorf

--
Martin Wessendorf, Ph.D.                       office:  (612) 626 0145
Assoc Prof, Dept Neuroscience                     lab:  (612) 624 2991
University of Minnesota                 Preferred FAX:  (612) 624 8118
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