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Hi Sarah-
        against my better judgement, I have looked at bacteria attached to
the bottom of plastic multi-well plates - Corning Costar 3603 - and got
beautiful images, to my surprise. I used our upright Nikon scopes and Leica
confocal, and turned the plates upside down. For those that attached to
glass, I used Whatman glass-bottomed plates like you mentioned - and those
were the very nice images you would expect.

Betsey


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Betsey Pitts
Research Associate/Facilities Manager, Microscopy
The Center for Biofilm Engineering
http://www.erc.montana.edu
366 EPS Building, Montana State University
Bozeman, MT 59717

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Ph (406) 994-7813
Fax (406) 994-6098

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-----Original Message-----
From: Sarah Locknar [mailto:[log in to unmask]]
Sent: Wednesday, April 09, 2003 9:37 AM
To: [log in to unmask]
Subject: plastic on inverted scope


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Hi-
I have a couple of users who want to image cells grown in plastic microwell
plates on an inverted scope.  Of course, the plastic is too thick to image
through, but when I suggested that they grow them on glass coverslips they
replied that the cells don't stick as well and grow differently on similarly
coated glass.  Is it possible to buy microwell plates with thinner plastic
bottoms, and where would I buy such a thing?  In previous postings I got the
feeling that the only available ones had glass coverslip bottoms.  Also,
would the images suffer greatly when imaging through plastic versus glass?
Would a better option be to just get a longer-working distance lens? Thanks
in advance- Sarah

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Sarah Locknar, Ph.D.
Director, Neuroscience COBRE Imaging/Physiology Core
University of Vermont
802-656-0413
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