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Hi Sarah, if you decide to go with the glass you may want to try Nalgene
Nunc's website.  They have chambers specially designed for inverted
microscopes.  The coverslip is located on the bottom (glass) and you can get
them at the appropriate thickness for imaging (1.5).  There are many
different styles to choose from and they are designed for culturing cells.
There is also another company called MatTek Coorporation
(http://www.glass-bottom-dishes.com/) and they sell small plastic petri
dishes with cover slips (glass) on the bottom, which can be coated with
poly-l-lysine or uncoated.  Best of all, both are more the willing to send
you some samples!!  Both are fairly expensive, both serve their purpose.  As
an added note, we clean and reuse our glass bottom petri dishes!!  Good luck
and I hope this helps.

Cheers,
Heather

Heather Ford
Plant Cell Microscopist
Donald Danforth Plant Science Center
Integrated Microscopy Facility
975 Warson Rd.
St. Louis, MO 63132
P: 314.587.1263
F: 314.587.1361
email: [log in to unmask]
www.danforthcenter.org

> ----------
> From:         Sarah Locknar
> Reply To:     Confocal Microscopy List
> Sent:         Wednesday, April 9, 2003 10:37 AM
> To:   [log in to unmask]
> Subject:      plastic on inverted scope
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi-
> I have a couple of users who want to image cells grown in plastic
> microwell plates on an inverted scope.  Of course, the plastic is too
> thick to image through, but when I suggested that they grow them on
> glass coverslips they replied that the cells don't stick as well and
> grow differently on similarly coated glass.  Is it possible to buy
> microwell plates with thinner plastic bottoms, and where would I buy
> such a thing?  In previous postings I got the feeling that the only
> available ones had glass coverslip bottoms.  Also, would the images
> suffer greatly when imaging through plastic versus glass?  Would a
> better option be to just get a longer-working distance lens?
> Thanks in advance-
> Sarah
>
> ------------------------------------------------------------
> Sarah Locknar, Ph.D.
> Director, Neuroscience COBRE Imaging/Physiology Core
> University of Vermont
> 802-656-0413
> ------------------------------------------------------------
>
>