Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Sarah,
There are plastic coverslips that have been around for years that are used
by electron microscopists, so check appropriate catalogs.  I've also seen
them in other catalogs such as Fisher and VWR.  They were needed because the
users had to slide very thin sections for viewing of their cultured cells,
and glass does not slice thinly or any other way.
Good luck,
Carl

Carl A. Boswell
Dept. of Mol. Cell. Biology
Univ. of Arizona
(520) 626-8469
FAX (520) 621-3709
[log in to unmask]
----- Original Message -----
From: "Sarah Locknar" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Wednesday, April 09, 2003 8:37 AM
Subject: plastic on inverted scope


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi-
> I have a couple of users who want to image cells grown in plastic
> microwell plates on an inverted scope.  Of course, the plastic is too
> thick to image through, but when I suggested that they grow them on
> glass coverslips they replied that the cells don't stick as well and
> grow differently on similarly coated glass.  Is it possible to buy
> microwell plates with thinner plastic bottoms, and where would I buy
> such a thing?  In previous postings I got the feeling that the only
> available ones had glass coverslip bottoms.  Also, would the images
> suffer greatly when imaging through plastic versus glass?  Would a
> better option be to just get a longer-working distance lens?
> Thanks in advance-
> Sarah
>
> ------------------------------------------------------------
> Sarah Locknar, Ph.D.
> Director, Neuroscience COBRE Imaging/Physiology Core
> University of Vermont
> 802-656-0413
> ------------------------------------------------------------