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Hi,
We have 2 Zeiss LSM systems and certainly we had the same problem.
Sometimes we see a message saying: 'hardware busy for more than 10 secs
and sometime is rotten.........'. I am sure a programmer had fun while
writing the codes.
Here are some things we do to minimize the problem:
1) Be patient. If a command needs time to finish (such as a hardware
movement), wait for it to finish. Try not clicking all over the places.
If you do, sooner or later, there will be some problem start to occur.
2) When you load configuration or hit "reuse" button, be patient and let
the system finish what they need to do.
3) Try to minimize software/services on the computer.
4) close unnecessary image windows.
It seems is is caused by a hardware which times out. But I suspect it is
rather a computer/software issue. One of our systems, we had frequently
such problem (the error log was about 1Mb in 3 yr. period!). But after
we upgraded the computer, it has been running for almost 2 yrs with
rarely the same problem.
Talk about Zeiss system, our collimators keeps timing out and stuck. Anyone
has
similar problem? Please email me privately if you think it is not
generally interesting. I am more interested what you can do to get rid
of the problem (beside asking Zeiss guys to come over and reset it
manually).
Thanks!
xuejun
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>Search the CONFOCAL archive at
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>Hi Alejandro,
>
>this is very interesting....
>I have a Zeiss LSM 510 mounted on an Axiovert 200M and recently we have
>been doing a lot of simultaneous DIC and fluorescent imaging and getting a
>similar problem to you - except that in our case it always says that the
>hardware was busy for more than 5 seconds! Clearly our hardware is even
>less happy at being busy for long periods of time than yours...
>
>Just for the record, we are on vs. 3.2 of the software, so if your Zeiss
>rep ever suggests that upgrading the software will help I'm afraid it
>probably won't. Sometimes it's enough to re-boot the software, sometimes
>we have to switch the microscope off and on, and other times we have to
>restart the whole computer to get things to work smoothly again.
>
>Are there others out there who have encountered the same problem
>too? Please let us know - I'd like to get this resolved too. Any comments
>from the Zeiss team???
>
>Best wishes,
>
>Alison
>
>
>
>At 09:22 AM 4/9/2003 -0700, you wrote:
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>Greetings to all<?xml:namespace prefix = o ns =
>>"urn:schemas-microsoft-com:office:office" />
>>
>>
>>
>>I am starting the operation of a LSM5 Pascal mounted on an Axiovert 200M
>>with the 2.8 version of LSM5 program. While the microscope can normally
>>respond, it sometimes develops trouble when going from visible into
>>Scanning mode. If this hapens, the programfreezes up and will not permit
>>any operation (opens a “switch to” or “retry” window). After a while, it
>>unfreezes, opens an error log window (Object: CscanController: Hardware
>>busy for more than 6 sec……… Object: ClsmObject: FW1 is busy), and begins
>>to scan. The local Zeiss representative was very surprised and is looking
>>for help on his end, but I have a line of people waiting to use the
equipment!
>>
>>I welcome all comments towards resolving this problem!
>>
>>
>>Thank you
>>
>>Alejandro D. Roth PhD
>>
>>FONDAP Center for Cell Regulation and Pathology
>>
>>Faculty of Biological Science
>>
>>Catholic University of Chile
>
>Dr. Alison J. North
>Rockefeller University,
>Bio-Imaging Resource Center, Box #209,
>1230 York Avenue,
>New York,
>NY 10021,
>USA
>
>Tel. (212) 327 7488
>Fax. (212) 327 7489
>e-mail: [log in to unmask]
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