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Hi Martyn

ImageJ will add text to the movie as you ask with the "Time Stamper" plugin (http://rsb.info.nih.gov/ij/plugins/stamper.html). The plugin asks you to enter starting time, time between frames and how many decimal points you'd like - by setting starting time to 10, time between frames to -0.1 and decimal point to zero, it should count down as you ask - unless I've got the maths wrong...

I can send you a tweaked version that uses antialiased text. One thing to remember is to convert your pseudocolour image to RGB before adding antialiased text - otherwise the "grey" pixels at the edges of curves/diagonals of white text on black background are psudocoloured giving the text a weird pseudocoloured halo.

I've also got a plugin that will add text to defined frames, e.g. "agonist on" starting frame X and stopping frame Y. Let me know if you'd like it.

Regards,

Tony



>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> We have used PaintShop Pro, but you cannot easily add the same text to
> multiple images. Can these other movie makers do this? so eg.
> allow the
> annotation to count down 5,4,3 etc sec to agonist in a movie of
> agonist-evoked Ca waves, which would require text to be added
> to blocks of
> 10 images if your rate is 10Hz.
>
> Thanks for any feedback
>
> Martyn Mahaut-Smith
> Dept of Physiology
> University of Cambridge
>
>
>


Tony Collins Ph.D.
Senior Research Associate
Calcium Group
Laboratory of Molecular Signalling
Babraham Institute
Cambridge, UK, CB2 4AT
Tel: +44 (0)1223 496499
Fax: +44 (0)1223 496043

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