Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi Pauline,
I'm not sure what you mean by "unprofessional"? ImageJ won't do video transitions, although, to be honest I haven't tried. For home movies of the kids I use the free Windows XP Movie maker (http://www.microsoft.com/windowsxp/moviemaker/downloads/moviemaker2.asp) which does do transitions - you can make the kids explode and all sorts...
It should work with microscopy movies too. For timelapse microscopy movies I find ImageJ does all I need it to.
If you want your movie to show your time-lapse experiment to put in to powerpoint or as a downloadable file then ImageJ is great (and free). It has plugins to export AVIs and quicktime, will timestamp each frame (antialased text, the font size and colour of your choice), event stamp designated frames, add scale bars of your choice, you can customise the LUT and framerate and it has the usual raft of filters.
If you want something more fancy (e.g. a graph of intensity vs time appearing along-side and in-sync with the movie), I find Macromedia FlashMX excellent (importing of movies was introduced with FlashMX v6). Not least because of the large, helpful web-community associated with Flash. Flash will import AVIs (maybe MOV - haven't tried) allowing you greater control of the movie, e.g. use keyboard arrow keys to scroll back and forth through the movie; stop, start, end buttons; adjustable frame rate etc. I'm no expert with it and have achieved this with help from the on-line community. Flash will also export AVI's. I think you can get an "educational" version which is fairly cheap.
Email me off list if you need help with any of this.
Regards
Tony
Senior Research Associate
Laboratory of Molecular Signalling
Babraham Institute
Babraham
Cambridge, UK
CB2 4AT
Tel: +44 (0)1223 496499
Fax: +44 (0)1223 496043
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-----Original Message-----
From: pauline yu
To: [log in to unmask]
Sent: 24/06/03 18:25
Subject: Re: making movies
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Dear Dr. Opas,
I just found an article posted online the other day which gave a basic
overview of making confocal movies (and featured some rather stunning
examples of their work).
It was from the Carroll lab homepage at UWisc
http://www.molbio.wisc.edu/carroll/movies.html
There is a link to the article at the bottom of the page.
I myself have used ImageJ and Confocal Assistant, but the results are
somewhat "unprofessional".
Good luck,
Pauline Yu
[log in to unmask]
----- Original Message -----
From: Michal Opas <[log in to unmask]>
Date: Tuesday, June 24, 2003 8:53 am
Subject: making movies
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello All,
>
> I searched the archive for "movies" and, interestingly, the newest
message
> was from 1966. So, I hope maybe someone will have newer answers.
> We want to make movies from strings of single sequential images saved
to a
> directory. This can be result of any experiment, in this particular
case
> it is circa 1300 of sequential video (TIF) images saved by "a
> time-lapsing" computer.
>
> Any suggestions regarding appropriate software would be welcome.
>
> Cheers
> Michal
>
>
>
> Dr. Michal Opas
> Department of Laboratory Medicine and Pathobiology
> University of Toronto
> 1 King's College Circle
> Medical Sciences Building, room 6326
> Toronto, Ontario, M5S 1A8 Canada
> --------------
> phone: (416) 978-8947 (laboratory)
> (416) 971-2140 (office)
> fax: (416) 978-5959
> email: [log in to unmask]
> homepage: http://www.utoronto.ca/mocell/index.html
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