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June 2003

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Subject:
blue diode laser for DAPI
From:
Leigh Silvester <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 5 Jun 2003 09:21:59 +0100
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Search the CONFOCAL archive at
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We have an infra-red laser that we use to excite Hoechst and DAPI  (multi-photon at 770 nm) but find the infra-red a bit tempremental and probably a bit expensive for this on a routine basis.

We are considering a 405 nm blue diode laser to study these fluorochromes.

Has anybody got any expereince of how good these lasers are for this work and what else such a laser may be good for?



Regards

Leigh Silvester
Division of Animal Physiology
University of Nottingham
School of Biosciences
Sutton Bonington Campus
Loughborough
Leicestershire
LE12 5RD, UK
tel: +44 (0)115 9515151 x18736
fax +44 (0)115 9516302
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