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November 2003

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Subject:
From:
Bangar Raju <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Sat, 1 Nov 2003 11:25:26 -0500
Content-Type:
text/plain
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Peter,

1. Wait for the arc stabilize before starting all
other electronic equipment including the computer.

2. Properly shield (using Aluminium Foil) the high
voltage cables from the igniter/power supply to the
lamp.

We have been successful in resolving the problems with
the RF sparks by following the above two procedures.

All the best.

Bangar
==================
Dr. Bangar R. Balajepalli
Technical Support Specialist
Instrument Division
Nikon Canada Inc.
Richmond, BC CANADA
==========================
           CONFOCAL Digest - 30 Oct 2003 to 31 Oct
2003 (#2003-243)
Table of contents:
   Trouble with Xenon Arc (2)
   hi fgrom singapore
   X-Cite 120
   Nikon PCM2000

   Trouble with Xenon Arc
      Trouble with Xenon Arc (10/31)
From: Peter Van Osta <[log in to unmask]>
      Re: Trouble with Xenon Arc (10/31)
From: Barbara Foster <[log in to unmask]>

   hi fgrom singapore
      Re: hi fgrom singapore (10/31)
From: aneez ahmed <[log in to unmask]>

   X-Cite 120
      X-Cite 120 (10/30)
From: Mayandi Sivaguru <[log in to unmask]>

   Nikon PCM2000
      Re: Nikon PCM2000 (10/31)
From: Richard Edelmann <[log in to unmask]>



---------------------------------
Browse the CONFOCAL online archives.> Date:    Fri, 31
Oct 2003 09:47:25 +0100
> From:    Peter Van Osta <[log in to unmask]>
> Subject: Trouble with Xenon Arc
>
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi,
>
> We are currently testing our new system in which we
> use a 75 W Xenon Arc
> (Carl Zeiss). We have developed a remote control
> system through which we
> can control the the power (unipolar switches) from
> within our software.
>
> Although we got the message that the Xenon Arc is
> properly shielded it
> frequently brings other electronic components in the
> system in disarray
> and this leads to malfunction of the entire system.
>
> We have tried netfilters and grounding but up to now
> we have not been
> able to protect our other equipment from periodic
> failure due to the
> spikes in the electricity net and/or the RF
> generated by the the Xenon Arc.
>
> We take care to schedule the powerup of the Xenon
> Arc before all other
> equipment and wait for 15 sec. before proceeding
> with the powerup
> sequence. Powerdown is in reverse order and the
> Xenon Arc is powered
> down as the last device.
>
> What can we do to solve this problem ?
>
> Best regards,
>
> Peter Van Osta
>
> Union Biometrica N.V./S.A.
> European Scientific Operations (ESO)
> Cipalstraat 3
> B-2440 Geel
> Belgium
> Tel.: +32 (0)14 570 620
> Fax.: +32 (0)14 570 621
>
> http://www.unionbio-eu.com/
>
>
http://ourworld.compuserve.com/homepages/pvosta/cvwww.htm
> > Date:    Fri, 31 Oct 2003 01:31:27 -0800
> From:    aneez ahmed <[log in to unmask]>
> Subject: Re: hi fgrom singapore
>
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> hi siva,
>
> how are u ? is this the same person.let me know i
> will
> reply u in detail
>
> aneez
>
>
> __________________________________
> Do you Yahoo!?
> Exclusive Video Premiere - Britney Spears
> http://launch.yahoo.com/promos/britneyspears/
> > Date:    Fri, 31 Oct 2003 08:57:40 -0800
> From:    Barbara Foster <[log in to unmask]>
> Subject: Re: Trouble with Xenon Arc
>
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear Peter,
>
> Have you tried the stablized system from OptiQuip?
> It might solve your
> problem.
>
> Best regards,
> Barbara Foster
> Microscopy/Microscopy Education
> 125 Paridon Street, Suite 102
> Springfield, MA 01118
> PH: 413-746-6931  FX: 413-746-9311  Web:
> www.MicroscopyEducation.com
>
> ~@~@~~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@
> Optimizing Light Microscopy for Biological and
> Clinical Labs is available
> in individual copies or classroom size orders.
> Visit
> www.MicroscopyEducation.com
> for details.
> ~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@~@
>
>
> At 09:47 AM 10/31/03 +0100, Peter Van Osta wrote:
> >Search the CONFOCAL archive at
>
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> >Hi,
> >
> >We are currently testing our new system in which we
> use a 75 W Xenon Arc
> >(Carl Zeiss). We have developed a remote control
> system through which we
> >can control the the power (unipolar switches) from
> within our software.
> >
> >Although we got the message that the Xenon Arc is
> properly shielded it
> >frequently brings other electronic components in
> the system in disarray
> >and this leads to malfunction of the entire system.
> >
> >We have tried netfilters and grounding but up to
> now we have not been
> >able to protect our other equipment from periodic
> failure due to the
> >spikes in the electricity net and/or the RF
> generated by the the Xenon Arc.
> >
> >We take care to schedule the powerup of the Xenon
> Arc before all other
> >equipment and wait for 15 sec. before proceeding
> with the powerup
> >sequence. Powerdown is in reverse order and the
> Xenon Arc is powered
> >down as the last device.
> >
> >What can we do to solve this problem ?
> >
> >Best regards,
> >
> >Peter Van Osta
> >
> >Union Biometrica N.V./S.A.
> >European Scientific Operations (ESO)
> >Cipalstraat 3
> >B-2440 Geel
> >Belgium
> >Tel.: +32 (0)14 570 620
> >Fax.: +32 (0)14 570 621
> >
> >http://www.unionbio-eu.com/
> >
>
>http://ourworld.compuserve.com/homepages/pvosta/cvwww.htm
> > Date:    Thu, 30 Oct 2003 21:52:41 -0600
> From:    Mayandi Sivaguru <[log in to unmask]>
> Subject: X-Cite 120
>
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Listers, I would like to get some insight on anybody
> who is currently using
> the fluorescence illumination system from the EXFO
> company's X-Cite 120
> (Exfo.com) particularly on the following aspects.
> Ease of use, User friendliness, Stability of the
> lamp intensity, life of
> the lamp and the liquid light guide and the cost to
> efficiency ratio or any
> relevant information on this product from the users.
> Although some of this
> information already I am aware of from the
> manufacturer, but would like to
> hear from somebody who is currently using it and
> having hands on experience.
> Thanks in advance
> Shiv
>
> Mayandi Sivaguru, Ph.D.,
> Associate Director
> Molecular Cytology Core Facility
> Molecular Biology Program
> 2, Tucker Hall
> University of Missouri
> Columbia, MO 65211-7400
> [log in to unmask]
>
> Voice: 1-573-882-4895
> Fax: 1-573-882-0123
>
> www.biotech.missouri.edu/mcc/
> > Date:    Fri, 31 Oct 2003 11:47:40 -0500
> From:    Richard Edelmann <[log in to unmask]>
> Subject: Re: Nikon PCM2000
>
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dave:
>
>         Since I did not see a response to this on
> the list.  Yes, the RGB are band
> pass fiters in a multi-color arrangement for the
> 488/543/633 laser lines.
>
>  The 505, 475 are a 505 Long Pass (LP) and 475 LP,
> the 475 is for using the
> 457 line off the Ar Ion laser, and Since multiline
> filter are less effcient the
> 505LP is for higher through put with just 488Line
> (i.e. for FITC or GFP only
> work).
>
> 565/RF/and HM are for spliting channel 1 and channel
> 2 apart:
>
> 565 below 565nm goes to channel 1, and above goes to
> channel 2.
>
> RF sends 100% of the emission light to channel 1.
>
> HM splits the emmision light into 50% channel 1 and
> 50% channel 2 (NO
> wavelength discrimination.)
>
> Yes I have a schemative of head and will send it
> along in a bit.
>
>
>
>
> > Can any PCM 2000 users out there tell me exactly
> what the prism settings on the
> > scanning head marked 'RGB, 505, 475' and 'RF, 565,
> HM' actually do? I assume the
> > RGB and numbered settings isolate single
> wavelengths from multiline lasers - but
> > the RF and HM? Does anyone have a schematic of the
> scanning head? I've asked
> > Nikon, but didn;t get anywhere.
> >
> > Thanks in advance
> >
> > Dave
> >
> > ----------------------------------------
> > David Corry
> > Centre for Research in Biomedicine
> > Faculty of Applied Sciences
> > University of the West of England
> > 0117 328 3397
> > Email: [log in to unmask]
> >
> >
> > --- End Forwarded Message ---
> >
> >
> > ----------------------------------------
> > David Corry
> > Centre for Research in Biomedicine
> > Faculty of Applied Sciences
> > University of the West of England
> > 0117 328 3397
> > Email: [log in to unmask]
> >
>
>
>
> Richard E. Edelmann, Ph.D.
> Electron Microscopy Facility Supervisor
> 350 Pearson Hall
> Miami University, Oxford, OH 45056
> Ph: 513.529.5712        Fax: 513.529.4243
> E-mail: [log in to unmask]
> http://www.emf.muohio.edu
>
> "RAM disk is NOT an installation procedure."
>

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