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Mike
I tend to agree with your assessment ( slider is defective) as the
system used to work prior to Leica replacing a slider. What I do not
understand is how this happens. What is more disturbing is the
possibility that incorrect spectra will be achieved by this mechanical
defect.  Since the Leica has three different channels and we can use the
same dichroic or reflector, the only difference is in the quality of the
PMT. That should not be that great so we can actually measure the
optical clarity of the system. In this case we observed spectra with
jagged edges in one of the PMT. We could have misinterpreted the data to
mean that we had some really unique spectra on a biological sample and
more frightening published those results as a great observation.. I
guess this means that we now need a test to make sure our sliders are
working properly.
Thanks for your input.
Bob

Robert M. Zucker, PhD
U.S. Environmental Protection Agency
Office of Research and Development
National Health and Environmental Effects Research Laboratory
Reproductive Toxicology Division, MD 72
Research Triangle Park, North Carolina, 27711
Tel: 919-541-1585; fax 919-541-4017
e-mail: [log in to unmask]




                      Michael Cammer
                      <[log in to unmask]        To:       Robert Zucker/RTP/USEPA/US@EPA
                      edu>                     cc:
                                               Subject:  Re: Spectral Problems
                      11/19/03 07:23 AM






spectra of the PMTs are different according to our service rep.  but
this
shouldn;t be the problem; I think the slits aren't stepping properly and
you need to have the mechanism fixed.


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