Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Dear,
Just a theoretical remark
We can state that HT is not done during human observation but is pure based
on machine vision. In theory, when no financial restrictions are present we
always can paralellize a system and reconstructing the image afterward. If
we like to evaluate photon emissions the limit to speed is the total amount
of photons needed to have a good signal to noise ratio. The limit for speed
is not realy a technological problem as scanning systems or whatever, the
limit is the signal. You need to collect a necessary amount of photons, and
if the sample emits these only at moderate intensity you are limited in
speed. In that way we can have forward you to the discussion on real time
imaging etc...
Bye
Patrick
----- Original Message -----
From: "Michael C. Adams" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Friday, January 16, 2004 9:46 PM
Subject: Re: High Throughput Microscopy Courses
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> *NO COMMERCIAL INTEREST*
>
> Relating to Barbara's comments about new equipment, I saw an HT-Confocal
> Microscopy setup that Atto is producing that looks quite nice and can scan
> several different kinds of plates. Looks interesting...
>
> Mike
>
> >===== Original Message From Confocal Microscopy List
> <[log in to unmask]> =====
> >Search the CONFOCAL archive at
> >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> >Hi, Carol,
> >
> >Actually, confocal has been very well adapted to reading microarrays in
> >which a number of "spots" of reactive material have been deposited on a
> >slide (Affymetrix does slides with up to 10,000 test spots). The sample
is
> >very different than most of us are used to seeing.
> >
> >Also, in addition to the more traditional microarrays, there are now
arrays
> >(typically 10-15 sites) onto which either cells or tissue have been
> deposited.
> >
> >In the SPM arena, they using silicon disks (3" blank wafers) and laying
> >down arrays of about 16-36 "spots" of materials.
> >
> >Scanning microscopy is taking on a whole new meaning!
> >
> >Best regards,
> > Barbara Foster
> >Microscopy/Microscopy Education
> >125 Paridon Street, Suite 102
> >Springfield, MA 01118
> >PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
> >
> >^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^
> >Don't forget: the American Chem Society's Applied Optical Microscopy
> >Course, March 5-7, Chicago, Ill.
> >Visit our website for details and registration. NOT JUST FOR
CHEMISTS!!!!
> >^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^
> >At 02:51 PM 1/16/04 -0800, Carol Heckman wrote:
> >>Search the CONFOCAL archive at
> >>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>
> >>Scott-
> >>Just on theoretical grounds, any of the scanning technologies would
> >>be difficult to adapt to high-throughput. For one thing, rastering
> >>takes time. Even with piezo technologies of moving the probe, a
> >>finite and largish amount of time is required to make up the "scene"
> >>for viewing. Collecting the whole image simultaneously is a much
> >>superior approach, if you want to view a lot of samples
> >>fast.
> >>
> >>Just a thought,
> >>Carol
> >>
> >>>Search the CONFOCAL archive at
> >>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>>
> >>>We don't run courses on "high throughput microscopy" as
> >>>such - do you really mean "high throughput"?
> >>>
> >>>we have run several graduate courses for many years at
> >>>Purdue. We are in our 11 consecutive year of offering these
> >>>courses. The Engineering courses were introduced 3 years
> >>>ago and we teach several courses that are designed
> >>>specifically for engineering students going into our
> >>>biomedical engineering program, many of whom don't have
> >>>strong bio backgrounds.
> >>>
> >>>BMS 524: Introduction to Confocal Microscopy and Image
> >>>analysis (1CR)
> >>>BMS 527: Practical component of above course (1CR)
> >>>BMS 633: Confocal microscopy and Image analysis for
> >>>Engineering students (2CR) ( includes practical)
> >>>
> >>>You can have all my lectures in PPT format if you want. I
> >>>have openly shared them for many years.
> >>>
> >>>Paul Robinson
> >>>Purdue
> >>>
> >>>
> >>>
> >>>On 15 Jan 2004 at 13:07, Scott Snyder wrote:
> >>>
> >>> Search the CONFOCAL archive at
> >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>>
> >>> I am trying to find out just how many courses covering hands on high
> >>> throughput microscopy are available to students as part of their
> >>> coursework.
> >>> Does anyone know of one of these, where and when it is held? I
> >>>know these are
> >>> pretty rare, I just want to find out how rare.
> >>>
> >>> Sincerely,
> >>>
> >>> D. Scott Snyder, Ph.D.
> >>> Lab Manager, Integrated Microscopy Core
> >>> Baylor College of Medicine
> >>> (713) 798-4952
> >>>
> >>>
> >>>J.Paul Robinson, PhD PH:(765)4940757
> >>>Professor of Immunopharmacology
> >>>Professor of Biomedical Engineering
> >>>Purdue University FAX:(765)4940517
> >>>EMAIL:[log in to unmask]
> >>>WEB: http://www.cyto.purdue.edu
> >>
> >>--
> >>__
> >>Carol A. Heckman, Ph.D.
> >>Professor of Biological Sciences
> >>Director, Center for Microscopy & Microanalysis
> >>Bowling Green State University, Bowling Green, OH 43403
> >>fax: (419) 372-2024 email: [log in to unmask]
>
>>__________________________________________________________________________
_
>
> >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
> Michael C. Adams
> Microscopy & Imaging Manager for Dr. Clare Waterman-Storer
> Laboratory of Cell Motility Studies
> Department of Cell Biology
> The Scripps Research Institute
> Attn: Mail Code CB-163
> 10550 North Torrey Pines Road
> La Jolla, CA 92037
>
> TEL 858.784.9244
> FAX 858.784.7521
> EMAIL [log in to unmask]
> >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
>
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