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Hi Gabor,

As you said, most segmentation and tracking software assumes bright features on a dark background (or vice versa), which causes problems for DIC images.

AutoDeblur has a DIC Restoration function that converts DIC images to an optical path length representation.  The results show cells as bright features on a dark background.  You can then use with your favourite segmentation and tracking software on the resulting image sequence.  A 30 day trial of AutoDeblur and the DIC Restoration is available at www.aqi.com.

AutoQuant also has a new Cell Counting and Tracking module that is currently in beta and will be released shortly.  

If anyone is interested in DIC processing, please feel free to contact me.

Cheers,
David
---
David Biggs, PhD
Senior Research Scientist
AutoQuant Imaging, Inc.
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-----Original Message-----
From: Csucs Gabor [mailto:[log in to unmask]]
Sent: Friday, January 23, 2004 5:33 AM
To: [log in to unmask]
Subject: Re: motion tracking


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Dear All

It is quite true that there are a number of softwares with which one can
perform tracking. My main problem is that for the segmentation of the
images these usually use tresholding which may work nicely for
fluorescent images but fails often when phase contrast images of cell
should be tracked. So my question is whether anyone is aware of a
software with which one can automatically track phase contrast (or DIC)
images of cells with minimal operator interference. Obviously in this
case only in 2D...

Thanks   Gabor

--
Gabor Csucs, PhD
Light Microscopy Center
Institute of Biochemistry
Swiss Federal Institute of Technology, ETHZ

tel     +41 1 633 6221
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