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DIAS has two different cell outline methods 
(gradient and threshold) and the gradient method 
works very well with DIC images.  Dave


>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi Gabor,
>
>As you said, most segmentation and tracking 
>software assumes bright features on a dark 
>background (or vice versa), which causes 
>problems for DIC images.
>
>AutoDeblur has a DIC Restoration function that 
>converts DIC images to an optical path length 
>representation.  The results show cells as 
>bright features on a dark background.  You can 
>then use with your favourite segmentation and 
>tracking software on the resulting image 
>sequence.  A 30 day trial of AutoDeblur and the 
>DIC Restoration is available at www.aqi.com.
>
>AutoQuant also has a new Cell Counting and 
>Tracking module that is currently in beta and 
>will be released shortly.
>
>If anyone is interested in DIC processing, please feel free to contact me.
>
>Cheers,
>David
>---
>David Biggs, PhD
>Senior Research Scientist
>AutoQuant Imaging, Inc.
>[log in to unmask]
>
>
>-----Original Message-----
>From: Csucs Gabor [mailto:[log in to unmask]]
>Sent: Friday, January 23, 2004 5:33 AM
>To: [log in to unmask]
>Subject: Re: motion tracking
>
>
>Search the CONFOCAL archive at
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>
>Dear All
>
>It is quite true that there are a number of softwares with which one can
>perform tracking. My main problem is that for the segmentation of the
>images these usually use tresholding which may work nicely for
>fluorescent images but fails often when phase contrast images of cell
>should be tracked. So my question is whether anyone is aware of a
>software with which one can automatically track phase contrast (or DIC)
>images of cells with minimal operator interference. Obviously in this
>case only in 2D...
>
>Thanks   Gabor
>
>--
>Gabor Csucs, PhD
>Light Microscopy Center
>Institute of Biochemistry
>Swiss Federal Institute of Technology, ETHZ
>
>tel     +41 1 633 6221
>         +41 1 633 6196                 ETH Hönngerberg
>fax     +41 1 633 1124                 CH-8093 Zürich
>email   [log in to unmask]
>www     http://www.biol.ethz.ch


--

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd.   U-3125
Storrs, CT 06269-3125
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860-486-2200      860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html