Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal As I indicated earlier, DIAS can track objects in 2D from DIC images. If you look at the images shown on http://www.geocities.com/solltech/dias/information/ you will see a DIC image of a cell being outlined by the software automatically. Dave -- Dr. David Knecht Department of Molecular and Cell Biology University of Connecticut 91 N. Eagleville Rd. U-3125 Storrs, CT 06269-3125 [log in to unmask] 860-486-2200 860-486-4331 (fax) home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html