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I think what you might be seeing is autofluorescence from mitochondria -
which are very regularly arranged in cardiac myocytes.

-----Original Message-----
From: Fredrik Swift [mailto:[log in to unmask]] 
Sent: 21 January 2004 10:16
To: [log in to unmask]
Subject: Cardiomyocytes


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Hello,

I'm labelling rat cardiomyocytes with different antibodies. Sometimes I
get what I think is unspecific staining. The staining consists of
longitudinal "strips" with low fluorescence intensity (Alexa-488
labelled 2nd ab's). This is typical if I use very low concentrations of
primary ab's, and I get similar results in negative control exp. with no
primary ab. Apparently, the 2ary ab is the cause of this, but I cannot
explain what it stains. Is it myosin/actin that I see? Have anyone had
the same results previously? Any help would be appreciated! I can send
jpg.-files of the images if the description wasn't good enough.

Regards,
Fredrik