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There really isn't too much trouble tracking moving objects in
phase or DIC provided nothing else is moving.  Just subtract each'
image from the previous one and changes are obvious - no need to
do any segmentation.

                                                    Guy Cox



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> >Dear All
> >
> >It is quite true that there are a number of softwares with which one can
> >perform tracking. My main problem is that for the segmentation of the
> >images these usually use tresholding which may work nicely for
> >fluorescent images but fails often when phase contrast images of cell
> >should be tracked. So my question is whether anyone is aware of a
> >software with which one can automatically track phase contrast (or DIC)
> >images of cells with minimal operator interference. Obviously in this
> >case only in 2D...
> >
> >Thanks   Gabor
> >
> >--
> >Gabor Csucs, PhD
> >Light Microscopy Center
> >Institute of Biochemistry
> >Swiss Federal Institute of Technology, ETHZ
> >
> >tel     +41 1 633 6221
> >        +41 1 633 6196                 ETH Hönngerberg
> >fax     +41 1 633 1124                 CH-8093 Zürich
> >email   [log in to unmask]
> >www     http://www.biol.ethz.ch
>
>
> Dear Gabor,
>
> I am not aware of such software.
>
> My suggestion is that you don't use Phase-Contrast (or DIC for that
> matter).
>
> If you want a single image that will show you RI
> changes, use dark field.  I know that doing this
> as NA >1 is difficult but I think that you will
> find that an NA 1.0 lens will give you a better
> and more easily interpretable image in dark field
> than a 1.2 NA phase of DIC setup on most living
> specimens.
>
> (doesn't work well on thick specimens with a lot
> of "out-of-focus" scattered light.)
>
> Jim P.
>
> --
>                ****************************************
> Prof. James B. Pawley,                             Ph.  608-263-3147
> Room 223, Zoology Research Building,               FAX  608-265-5315
> 1117 Johnson Ave., Madison, WI, 53706  [log in to unmask]
> "A scientist is not one who can answer questions but one who can
> question answers."  Theodore Schick Jr., Skeptical Enquirer, 21-2:39
>


--
Associate Professor Guy Cox
Electron Microscope Unit, F09
University of Sydney NSW 2006
+61 2 9351 3176