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Regarding DF at NA>1.0, be sure to use an oil objective with a built-in
iris.

Does anyone know anything about "Virtual Microscopy" software products?
Which one works best? Any suggestions?  Thanks,

Al


>From: James Pawley <[log in to unmask]>
>Reply-To: Confocal Microscopy List <[log in to unmask]>
>To: [log in to unmask]
>Subject: Re: motion tracking
>Date: Sun, 25 Jan 2004 19:02:18 -0600
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>Dear All
>>
>>It is quite true that there are a number of softwares with which one can
>>perform tracking. My main problem is that for the segmentation of the
>>images these usually use tresholding which may work nicely for
>>fluorescent images but fails often when phase contrast images of cell
>>should be tracked. So my question is whether anyone is aware of a
>>software with which one can automatically track phase contrast (or DIC)
>>images of cells with minimal operator interference. Obviously in this
>>case only in 2D...
>>
>>Thanks   Gabor
>>
>>--
>>Gabor Csucs, PhD
>>Light Microscopy Center
>>Institute of Biochemistry
>>Swiss Federal Institute of Technology, ETHZ
>>
>>tel     +41 1 633 6221
>>        +41 1 633 6196                 ETH Hönngerberg
>>fax     +41 1 633 1124                 CH-8093 Zürich
>>email   [log in to unmask]
>>www     http://www.biol.ethz.ch
>
>
>Dear Gabor,
>
>I am not aware of such software.
>
>My suggestion is that you don't use Phase-Contrast (or DIC for that
>matter).
>
>If you want a single image that will show you RI changes, use dark field.
>I know that doing this as NA >1 is difficult but I think that you will find
>that an NA 1.0 lens will give you a better and more easily interpretable
>image in dark field than a 1.2 NA phase of DIC setup on most living
>specimens.
>
>(doesn't work well on thick specimens with a lot of "out-of-focus"
>scattered light.)
>
>Jim P.
>
>--
>               ****************************************
>Prof. James B. Pawley,                             Ph.  608-263-3147
>Room 223, Zoology Research Building,               FAX  608-265-5315
>1117 Johnson Ave., Madison, WI, 53706  [log in to unmask]
>"A scientist is not one who can answer questions but one who can
>question answers."  Theodore Schick Jr., Skeptical Enquirer, 21-2:39

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