Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello Louis,

On the the transfection of GFP-actin. You should read the papers published
by Andrew Matus (Zurich, Switzerland)
using a gamma-actin construct fused to GFP under a beta-actin promoter. They
have used this construct to label the
dynamic changes in spine heads in neurons. We have used this construct and
it works well.

Hope this helps,

Greg

Gregory A. O'Sullivan PhD
Deutschordenstrasse 46
60528 Frankfurt
Deutschland
Tel: +49 69 96769315
Fax: +49 69 96769441
Mob: +49 176 21179530
email: [log in to unmask]

----- Original Message -----
From: "Louis Villeneuve" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Monday, May 31, 2004 10:52 PM
Subject: F-Actin staining in living cells


Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Bonjour,

I want to stain F-Actin in living myo-fibroblast (in culture).  what would
be the reagent of choice?  Of course, I want it to be non toxic and membrane
permeable.

Any ideas?

Thanks for the answers!!!

Louis
Louis R. Villeneuve
Assistant de recherche - microscope  confocal
Institut de Cardiologie de Montréal- Centre de Recherche
5000 est Bélanger, Montréal
Québec , Canada
H1T 1C8

514-376-3330 ext3511
514-376-1355 (Fax)