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You are not giving us enough information.  The signal should be
hugely brighter in the non-descanned detectors and in my experience
(using the MRC 1024 at the IGC) it always is.  BUT the placement of
the various filters in this detector is crucial and far from intuitive.
Please tell us exactly what filters you have and where (not just the
Olympus filter blocks but those other filters you have to put in
various inconvenient locations).

                                                    Guy


Quoting Robert Fernandez <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear all
>
> I am currently running a Biorad MRC1024 Confocal/Multiphoton with a TiS
> Tsunami multiphoton laser and a Kr/Ar multiline laser. Wehn we thry and
> image
> EGFP or FITC with our external (descaned) detectors, using the green/red or
> blue/green Olypus filter blocks, we receive very little signal. When we then
> use
> the internal detectors, we find that  the signal is nice and bright. We
> rarely use
> them, infact this is the first time in 8 months. Does anyone have any
> experience in using these, or any ideas what could be wrong with the
> external
> detectors?
>
> Thank you
>
> Robert Fernandez
> IMDR
> School Of Biological Sciences
> University Of Manchester
> {HYPERLINK "mailto:[log in to unmask]"}
>


--
Associate Professor Guy Cox
Electron Microscope Unit, F09
University of Sydney NSW 2006
+61 2 9351 3176