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Aleks,

You can count cells with ImageJ which is free. In ImageJ you set an
intensity threshold and specify the range of sizes you are interested
in; then it will count
the cells and report their parameters.

Michael Model
Confocal Imaging Core
Kent State University
tel. 330-672-2304

----- Original Message -----
From: "Spurmanis, Aleks" <[log in to unmask]>
Date: Friday, February 18, 2005 6:07 pm
Subject: cell counting

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi everyone,
>
> I'm looking for imaging software that can allow me to rapidly
> quantify the
> relative numbers of astrocytes (immuno-stained with AF488 tagged
> Abs to
> GFAP) and neurons (immuno-stained with AF546 tagged Abs to MAP2)
> in a
> primary culture of embryonic mouse cortical tissue.  I was
> planning to image
> the cells using WF epifluorescence and count the number of cells
> in both
> fluorescence channels in 3-4 visual fields using a low power
objective
> (e.g., 10x).  I believe that I could perform this analysis quite
> easily with
> Image Pro Plus, but was wondering if anyone could recommend a less
> expensivealternative (MACE?) that will still get the job done?  In
> addition, I
> welcome any comments or suggestions to improve my methodology (e.g.,
> switching to a multi-parameter fluorescent microplate assay).
>
> Thanks,
>
> Aleks Spurmanis
> Confocal Core Facility Manager
> Institute for Nutrisciences and Health
> 93 Mount Edward Road Suite M3-11
> Charlottetown PE
> C1A 5T1
> tel:  (902)- 566-7457
> fax: (902) - 569-4289
> e-mail:  [log in to unmask]
> <mailto:[log in to unmask]>
>
>