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Robert Zucker wrote:
> Are
> they any other test substrates that can be used to detect optical issues
> in a spectrometer based confocal microscope. Some of the manufactures of
> confocal microscopes have not accepted this lamp as a standard to QA
> their machines.
I'd suggest you just use the microscope laser lines. They can hardly
argue with that.
One question - is the signal at PMT3 with Airy 1 pinhole substantially
lower intensity as well as lower spectral resolution? One might expect
that if the cause is misalignment it would also make the signal weaker.
Guy
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Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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