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Hi Simon,
Start with the "Scan Multi-Well Plates" command (Devices -> Stage -> Scan
Multi-Well Plate [MM6, should be in similar locatioon in MM5). Best to test
using a single acquisition channel (i.e. phase contrast image -> stack)
before doing any eleaborate journals. Good to run a few times to check
repeatability, i.e. acquire to "stack1" (then save to a new filename like
SimonSays1) then repeat (save to SimonSays2), etc, and use Arithmetic, all
planes, 1 - 2 + offset, to see how the entire system performs.
If you need multiple channels, Z-series (or auto-focus), or to acquire
multiple fields within the well (move stage: relative), it is best to do the
"inner loop" steps at each position in a journal that meets your
experimental requirements (exposure time, binning, etc for each channel).
The "Scan multi-well plates"command can itself be journalized for use in
Acquire Timelapse.
Best wishes,
George
George McNamara, Ph.D.
Imaging Scientist
Congressman Julian Dixon Image Core
The Saban Research Institute of Childrens Hospital Los Angeles
4650 Sunset Blvd., MS 133, SRT 1016
Los Angeles, CA 90027
323-669-2548 voice
323-251-8878 cell
323-668-7921 fax
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http://shelia/image Image Core Web (CHLA Intranet)
\\ntapps49\home Image Core User Home (CHLA Intranet)
\\ntapps49\appnotes Image Core Application Notes (CHLA Intranet)
http://www.childrenshospitalla.org/research.html CHLA Saban Research
Institute
http://home.earthlink.net/~geomcnamara Personal web site
http://home.earthlink.net/~mpmicro Microscopy web
site
http://www.mcb.arizona.edu/ipc/fret/default.htm Fluorescent Spectra
site
"Old soldiers never die; they just fade away." - D. Macarthur.
"Old antibodies die; please throw them away." - GM.
-----Original Message-----
From: swatkins [mailto:[log in to unmask]]
Sent: Wednesday, February 16, 2005 7:56 AM
To: [log in to unmask]
Subject: Metamorph plate scanning journals
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Folks rather than reinvent the wheel, I was curious as to whether anyone has
written a decent plate scanning journal (96 well) for Metamorph?
Simon C. Watkins Ph.D. FRC Path.
Professor, Cell Biology and Physiology and Immunology
Director Graduate Program in Cell Biology and Physiology
Vice Chair, Cell Biology and Physiology
Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St.
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-2797
URL: http://www.cbi.pitt.edu
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