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I believe DPX will do. I used it with Cy3 which survived dehydration. But
you might need to test other fluorochromes if you want to use it.

Xuejun

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Guenter Giese
Sent: Tuesday, June 07, 2005 2:56 AM
To: [log in to unmask]
Subject: fluorescent sample embedding medium with high refractive index

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Dear list members,

which are the embedding media with highest refractive index (r.i. 1.48 to
1.52) but still compatible with standard fluorochromes / fluorescent
proteins?

Ideally, the refractive index would match the r.i .of immersion oil (e.g.
ZEISS Immersol 518 F: r.i. 1.518).

Any reference welcome.

Guenter

------------------------------------------
Dr. Guenter Giese
Light Microscopy Facility
Dept. of Biomedical Optics, MPI fuer Medizinische Forschung
Jahnstr. 29, D-69120 Heidelberg, Germany
Phone (+49) 6221-486-360 (Fax: -325)
e-mail: [log in to unmask]
http://www.mpimf-heidelberg.mpg.de/~ggiese/lightmicro


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