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Dear Simon,
Sigma sells PKH fluorescent cell linker dyes. They have been used in
a wide variety of applications (s. bibliography on web page).
A labeling protocol is posted there as well. The dye is actually
produced by Phanos Technologies.
The advantage of this dye is the low toxicity and long lifetime.

I hope this helps,
Kolja

PKH Fluorescent Cell Linker Dyes:
http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/
Antibody_Explorer/Technical_Support/PKH_Fluorescent_Cell.html

________________________
Kolja Wawrowsky
Confocal Core
Cedars-Sinai Medical Center
Davis-6065
Los Angeles, CA 90048

office: (310) 423-2349
lab: (310) 423-4429

On Jun 7, 2005, at 9:28 AM, Simon Walker wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Can anyone recommend a fluorescent dye (and suitable protocol) to
> label
> membranes (plasma membrane)in tissue (Drosophila eye).  The tissue is
> expressing GFP so something which emits red would be ideal.  I see
> Molecular Probes, sorry Invitrogen, sell their lipohpilic tracers
> (e.g.
> Dil) as crystals, oils and pastes but I'm not sure which would be
> best for
> this application.  Any advice gratefully received.
> Thanks
> Simon
>