Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Personally, if I'm doing anything that matters (ie
not using either oil or very low mag) I use a lens
with a correction collar.  And I take the trouble to
set it correctly!  The difference I get between a
normal .75 NA plan fluorite and a .95NA plan apo
is enormous, and it's not the improvement in NA, nor
the better colour correction, it's the ability to
correct SA.  Quicker, easier and more accurate than
measuring coverslips.  Well worth the cost (which in
any case is minor if ordered as part of the original
microscope)

                                    Guy


> As slight topic drift here but... In general do people bother to measure
> every coverslip with a micrometer before you use it or do people do
> something else, eg. measure a few from a box, or assume that #1.5 are
> 170um?
>
> Ian
>


--
Associate Professor Guy Cox
Electron Microscope Unit,
University of Sydney,
NSW 2006, Australia

Phone:+61 2 9351 3176    Fax:+61 2 9351 7682
http://www.guycox.net