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Guy- What procedure do you use to set the correction collar?  I have
always found this challenging due to the need to refocus as you
rotate.  I am usually trying to use it on our confocal system and I
have not sure whether DIC, widefield or confocal is the best way to
determine the right setting.    Dave

Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)

On Jun 10, 2005, at 1:45 PM, Guy Cox wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Personally, if I'm doing anything that matters (ie
> not using either oil or very low mag) I use a lens
> with a correction collar.  And I take the trouble to
> set it correctly!  The difference I get between a
> normal .75 NA plan fluorite and a .95NA plan apo
> is enormous, and it's not the improvement in NA, nor
> the better colour correction, it's the ability to
> correct SA.  Quicker, easier and more accurate than
> measuring coverslips.  Well worth the cost (which in
> any case is minor if ordered as part of the original
> microscope)
>
>                                     Guy
>
>
>
>> As slight topic drift here but... In general do people bother to
>> measure
>> every coverslip with a micrometer before you use it or do people do
>> something else, eg. measure a few from a box, or assume that #1.5 are
>> 170um?
>>
>> Ian
>>
>>
>
>
> --
> Associate Professor Guy Cox
> Electron Microscope Unit,
> University of Sydney,
> NSW 2006, Australia
>
> Phone:+61 2 9351 3176    Fax:+61 2 9351 7682
> http://www.guycox.net
>