CONFOCALMICROSCOPY Archives

June 2005

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
Re: CONFOCAL Digest - 12 Jun 2005 to 13 Jun 2005 (#2005-135)
From:
Kate Phelps <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 14 Jun 2005 11:54:20 -0500
Content-Type:
text/plain
Parts/Attachments:
text/plain (39 lines) 
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

----------------------------------------------------------------------

Date:    Mon, 13 Jun 2005 11:57:27 +0630
From:    Nandini Rangaraj <[log in to unmask]>
Subject: IMAGE J

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi ,
Does anybody know how to quantitate fluorescence in a dual image (ie
getting fluorescencs values for FITC and Texasred separately) using the
same ROI(region of interest) using IMAGE J?
Thanks in advance
Nandini
***************************************************************

As the others have noted you can separate the RGB into separate images. Then make an ROI on one and then you can use Edit>Selection>Selection Restore to copy the ROI to the other channel(s). The documentation for this is here:

http://rsb.info.nih.gov/ij/docs/menus/edit.html#selection

Kate



Kate Luby-Phelps, Ph.D.
Associate Professor
Department of Cell Biology
UT Southwestern Medical School
5323 Harry Hines Blvd.
Dallas, TX 75390-9039

T: 214-648-0429
F: 214-648-8649
Reply to: [log in to unmask]

ATOM RSS1 RSS2