Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

There's a bit about toxicity in a paper by Scaduto and Grotyohann:

Scaduto RC Jr, Grotyohann LW. Measurement of mitochondrial membrane
potential using fluorescent rhodamine derivatives. Biophys J. 1999
Jan;76(1 Pt 1):469-77. PMID: 9876159

There's also a nice chapter on 'monitoring mitochondrial function in
single cells' by Mojet et al. in 'Calcium Signalling - A practical
approach' Ed. Tepikin 2nd edition 2001. Oxford. This covers a range of
dyes, not just calcium-dyes. One of the sections is specfically on
toxity. They point out that DiOC6 is almost as toxic as rotenone!

My experience is that you can induce mitochondrial permeability
transition quite easily by imaging TMRE. This was useful to investigate
mitochondrial connectivity but not helpful for long term studies!

Regards,

Tony



Guy Cox wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> A query from a student at the EMBO Live Cell Imaging
> Course:  does anyone have information on the relative
> photoxicity of different mitochondrial dyes?
>
>                                       Guy
>
> --
> Associate Professor Guy Cox
> Electron Microscope Unit,
> University of Sydney,
> NSW 2006, Australia
>
> Phone:+61 2 9351 3176    Fax:+61 2 9351 7682
> http://www.guycox.net

--
Tony Collins, Ph.D.
Facility Manager
Wright Cell Imaging Facility
Toronto Western Research Institute
13-407 McLaughlin Pavilion
399 Bathurst Street
Toronto, ON. M5T 2S8
tel. (416) 603 5367 fax: (416) 603 5745
http://www.uhnresearch.ca/wcif