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Hi David,

try http://www.embl.de/eamnet/html/bleach_correction.html
its a simple imageJ macro. Select a region and the algorithm will keep the
intensity in this region constant.

Cheers, jens

Quoting "david.depoil" <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi fellow confocalists
>
> I have observed that my monitoring beam has been bleaching my flurochrome
> (Cy5).  Is there a known formula that relates the amount of bleaching to
> the number of images acquired (or at least a functional form)? (so that I
> may correct for this "background" bleaching)
>
> any help would be much appreciated!
>
> David Depoil
> PhD student
> Valitutti's Team
> U563 CPTP Inserm
> Toulouse
> France
>



--
Dr. Jens Rietdorf
EMBL, Meyerhofstr.1,
D-69117 Heidelberg,
Cell Biology/Biophysics Program,
Advanced Light Microscopy Facility
Phone ++49(0)6221 387-467 FAX-306
http://www.embl.de/almf